Hepatocyte specific model
Starting with the thermodynamically curated human genome-scale Recon 3D30,31, we reconstructed a hepatocyte metabolic model by taking into account the physiology of hepatocytes and the genes expressed in liver cells. Towards this end, we defined the physiology of hepatocytes by integrating in the human Recon3D model publicly available fluxomics data for 92 boundary reactions28 and metabolomics data for 213 metabolites29 from previous hepatocyte model reconstructions. Additionally, we set the growth rate of the hepatocyte to a maximum of 0.014 h−1 corresponding to a doubling time of 49.5 h49 and the ATP maintenance rate to at least 1.07 mmol/gDW/h50. The Human Protein Atlas (www.proteinatlas.org)27 was used to identify 1853 metabolic genes present in…
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